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Developmental Studies Hybridoma Bank mouse anti chicken mhc β antibody
Effects of dietary 25-hydroxycholecalciferol (25-OH-D3) supplementation on heart failure marker expressions of broiler breeder hens provided with restricted (R) or ad libitum (Ad) feed intake. Hearts collected at 28, 33, and 45 weeks were used for cardiac failure marker, BNP (brain natriuretic peptide), and <t>MHC-β</t> (myosin heavy chain, cardiac muscle beta) expressions through the qRT-PCR method with normalization to GADPH (n = 4 ( A )) and by immunohistochemistry (n = 3 ( B )). All results are expressed as ratios relative to R-hens at 28 or 33 weeks *; significant difference by Ad-feed intake (vs. corresponding R-hens, p < 0.05) +; significant difference by 25-OH-D3 (vs. R- or Ad-hens, p < 0.05).
Mouse Anti Chicken Mhc β Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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beta myosin heavy chain β mhc  (Proteintech)
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Effects of dietary 25-hydroxycholecalciferol (25-OH-D3) supplementation on heart failure marker expressions of broiler breeder hens provided with restricted (R) or ad libitum (Ad) feed intake. Hearts collected at 28, 33, and 45 weeks were used for cardiac failure marker, BNP (brain natriuretic peptide), and <t>MHC-β</t> (myosin heavy chain, cardiac muscle beta) expressions through the qRT-PCR method with normalization to GADPH (n = 4 ( A )) and by immunohistochemistry (n = 3 ( B )). All results are expressed as ratios relative to R-hens at 28 or 33 weeks *; significant difference by Ad-feed intake (vs. corresponding R-hens, p < 0.05) +; significant difference by 25-OH-D3 (vs. R- or Ad-hens, p < 0.05).
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Effects of dietary 25-hydroxycholecalciferol (25-OH-D3) supplementation on heart failure marker expressions of broiler breeder hens provided with restricted (R) or ad libitum (Ad) feed intake. Hearts collected at 28, 33, and 45 weeks were used for cardiac failure marker, BNP (brain natriuretic peptide), and <t>MHC-β</t> (myosin heavy chain, cardiac muscle beta) expressions through the qRT-PCR method with normalization to GADPH (n = 4 ( A )) and by immunohistochemistry (n = 3 ( B )). All results are expressed as ratios relative to R-hens at 28 or 33 weeks *; significant difference by Ad-feed intake (vs. corresponding R-hens, p < 0.05) +; significant difference by 25-OH-D3 (vs. R- or Ad-hens, p < 0.05).
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Effects of dietary 25-hydroxycholecalciferol (25-OH-D3) supplementation on heart failure marker expressions of broiler breeder hens provided with restricted (R) or ad libitum (Ad) feed intake. Hearts collected at 28, 33, and 45 weeks were used for cardiac failure marker, BNP (brain natriuretic peptide), and <t>MHC-β</t> (myosin heavy chain, cardiac muscle beta) expressions through the qRT-PCR method with normalization to GADPH (n = 4 ( A )) and by immunohistochemistry (n = 3 ( B )). All results are expressed as ratios relative to R-hens at 28 or 33 weeks *; significant difference by Ad-feed intake (vs. corresponding R-hens, p < 0.05) +; significant difference by 25-OH-D3 (vs. R- or Ad-hens, p < 0.05).
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Effects of dietary 25-hydroxycholecalciferol (25-OH-D3) supplementation on heart failure marker expressions of broiler breeder hens provided with restricted (R) or ad libitum (Ad) feed intake. Hearts collected at 28, 33, and 45 weeks were used for cardiac failure marker, BNP (brain natriuretic peptide), and <t>MHC-β</t> (myosin heavy chain, cardiac muscle beta) expressions through the qRT-PCR method with normalization to GADPH (n = 4 ( A )) and by immunohistochemistry (n = 3 ( B )). All results are expressed as ratios relative to R-hens at 28 or 33 weeks *; significant difference by Ad-feed intake (vs. corresponding R-hens, p < 0.05) +; significant difference by 25-OH-D3 (vs. R- or Ad-hens, p < 0.05).
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Effects of dietary 25-hydroxycholecalciferol (25-OH-D3) supplementation on heart failure marker expressions of broiler breeder hens provided with restricted (R) or ad libitum (Ad) feed intake. Hearts collected at 28, 33, and 45 weeks were used for cardiac failure marker, BNP (brain natriuretic peptide), and <t>MHC-β</t> (myosin heavy chain, cardiac muscle beta) expressions through the qRT-PCR method with normalization to GADPH (n = 4 ( A )) and by immunohistochemistry (n = 3 ( B )). All results are expressed as ratios relative to R-hens at 28 or 33 weeks *; significant difference by Ad-feed intake (vs. corresponding R-hens, p < 0.05) +; significant difference by 25-OH-D3 (vs. R- or Ad-hens, p < 0.05).
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Effects of dietary 25-hydroxycholecalciferol (25-OH-D3) supplementation on heart failure marker expressions of broiler breeder hens provided with restricted (R) or ad libitum (Ad) feed intake. Hearts collected at 28, 33, and 45 weeks were used for cardiac failure marker, BNP (brain natriuretic peptide), and <t>MHC-β</t> (myosin heavy chain, cardiac muscle beta) expressions through the qRT-PCR method with normalization to GADPH (n = 4 ( A )) and by immunohistochemistry (n = 3 ( B )). All results are expressed as ratios relative to R-hens at 28 or 33 weeks *; significant difference by Ad-feed intake (vs. corresponding R-hens, p < 0.05) +; significant difference by 25-OH-D3 (vs. R- or Ad-hens, p < 0.05).
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Effects of dietary 25-hydroxycholecalciferol (25-OH-D3) supplementation on heart failure marker expressions of broiler breeder hens provided with restricted (R) or ad libitum (Ad) feed intake. Hearts collected at 28, 33, and 45 weeks were used for cardiac failure marker, BNP (brain natriuretic peptide), and <t>MHC-β</t> (myosin heavy chain, cardiac muscle beta) expressions through the qRT-PCR method with normalization to GADPH (n = 4 ( A )) and by immunohistochemistry (n = 3 ( B )). All results are expressed as ratios relative to R-hens at 28 or 33 weeks *; significant difference by Ad-feed intake (vs. corresponding R-hens, p < 0.05) +; significant difference by 25-OH-D3 (vs. R- or Ad-hens, p < 0.05).
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Effects of dietary 25-hydroxycholecalciferol (25-OH-D3) supplementation on heart failure marker expressions of broiler breeder hens provided with restricted (R) or ad libitum (Ad) feed intake. Hearts collected at 28, 33, and 45 weeks were used for cardiac failure marker, BNP (brain natriuretic peptide), and MHC-β (myosin heavy chain, cardiac muscle beta) expressions through the qRT-PCR method with normalization to GADPH (n = 4 ( A )) and by immunohistochemistry (n = 3 ( B )). All results are expressed as ratios relative to R-hens at 28 or 33 weeks *; significant difference by Ad-feed intake (vs. corresponding R-hens, p < 0.05) +; significant difference by 25-OH-D3 (vs. R- or Ad-hens, p < 0.05).

Journal: Antioxidants

Article Title: 25-Hydroxycholecalciferol Improves Cardiac Metabolic Adaption, Mitochondrial Biogenetics, and Redox Status to Ameliorate Pathological Remodeling and Functional Failure in Obese Chickens

doi: 10.3390/antiox13111426

Figure Lengend Snippet: Effects of dietary 25-hydroxycholecalciferol (25-OH-D3) supplementation on heart failure marker expressions of broiler breeder hens provided with restricted (R) or ad libitum (Ad) feed intake. Hearts collected at 28, 33, and 45 weeks were used for cardiac failure marker, BNP (brain natriuretic peptide), and MHC-β (myosin heavy chain, cardiac muscle beta) expressions through the qRT-PCR method with normalization to GADPH (n = 4 ( A )) and by immunohistochemistry (n = 3 ( B )). All results are expressed as ratios relative to R-hens at 28 or 33 weeks *; significant difference by Ad-feed intake (vs. corresponding R-hens, p < 0.05) +; significant difference by 25-OH-D3 (vs. R- or Ad-hens, p < 0.05).

Article Snippet: An antigen retrieval method as described previously was used for immunohistochemical analysis with a mouse anti-chicken MHC-β antibody (clone 2E9, Developmental Studies Hybridoma Bank, Iowa City, IA, USA) to visualize MHC-β expression and an avian-specific mouse monoclonal antibody conjugated with FITC (Clone KUL01, Abcam) for macrophage infiltration [ , ].

Techniques: Marker, Quantitative RT-PCR, Immunohistochemistry